› 5nx0

X-ray diffraction
1.8Å resolution

Structure of Spin-labelled T4 lysozyme mutant L115C-R119C-R1 at room temperature

Released:
DOI: 10.2210/pdb5nx0/pdb
Model geometry
Fit model/data
PDB entry 5nx0 coloured by chain, front view.
PDB entry 5nx0 coloured by chain, side view.
PDB entry 5nx0 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Combining EPR spectroscopy and X-ray crystallography to elucidate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals.
Consentius P, Gohlke U, Loll B, Alings C, Heinemann U, Wahl MC, Risse T
Phys Chem Chem Phys 19 20723-20734 (2017)
PMID: 28740983

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.58 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D9IEF7 (Residues: 1-164; Coverage: 100%)
Gene names: T4Tp126, e
Sequence domains: Phage lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: P3221
Unit cell:
a: 61.075Å b: 61.075Å c: 97.066Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.158 0.209
Expression system: Escherichia coli BL21

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