Function and Biology Details
Reaction catalysed:
D-galactosyl-N-acylsphingosine + H(2)O = D-galactose + N-acylsphingosine
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
Saposin-A and Galactocerebrosidase (preferred)
PDBe Complex ID:
PDB-CPX-157109 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Galactocerebrosidase
Molecule details ›
Chains: A, B
Length: 654 amino acids
Theoretical weight: 74.6 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
Sequence domains:
Length: 654 amino acids
Theoretical weight: 74.6 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
- Canonical:
P54818 (Residues: 41-684; Coverage: 100%)
Sequence domains:
- Glycosyl hydrolase family 59
- Glycosyl hydrolase family 59 central domain
- Galactocerebrosidase, C-terminal lectin domain
Saposin-A
Molecule details ›
Chains: C, D
Length: 87 amino acids
Theoretical weight: 9.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains: Saposin-like
Length: 87 amino acids
Theoretical weight: 9.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
- Canonical: Q61207 (Residues: 59-143; Coverage: 16%)
Sequence domains:
Structure domains: Saposin-like
Ligands and Environments
Experiments and Validation Details
X-ray source:
DIAMOND BEAMLINE I04-1
Spacegroup:
P6222
Expression systems:
- Homo sapiens
- Escherichia coli
