› 5nxn

X-ray diffraction
3.12Å resolution

Structure of a L5-deletion mutant of Providencia stuartii Omp-Pst1

Released:
DOI: 10.2210/pdb5nxn/pdb
PDB entry 5nxn coloured by chain, front view.
PDB entry 5nxn coloured by chain, side view.
PDB entry 5nxn coloured by chain, top view.
Source organism: Providencia stuartii
Primary publication:
Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities.
El-Khatib M, Nasrallah C, Lopes J, Tran QT, Tetreau G, Basbous H, Fenel D, Gallet B, Lethier M, Bolla JM, Pagès JM, Vivaudou M, Weik M, Winterhalter M, Colletier JP
Proc Natl Acad Sci U S A 115 E2220-E2228 (2018)
PMID: 29476011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 342 amino acids
Theoretical weight: 37.88 KDa
Source organism: Providencia stuartii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: E3U904 (Residues: 23-374; Coverage: 91%)
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 126.321Å b: 158.985Å c: 101.326Å
α: 90° β: 124.65° γ: 90°
R-values:
R R work R free
0.247 0.246 0.268
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
1 more

1 mention without citation

Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities.
El-Khatib et al. (2018)