› 5nxr

X-ray diffraction
2.7Å resolution

Trimeric structure of Omp-Pst1, the major porin from Providencia stuartii

Released:
DOI: 10.2210/pdb5nxr/pdb
PDB entry 5nxr coloured by chain, front view.
PDB entry 5nxr coloured by chain, side view.
PDB entry 5nxr coloured by chain, top view.
Source organism: Providencia stuartii
Primary publication:
Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities.
El-Khatib M, Nasrallah C, Lopes J, Tran QT, Tetreau G, Basbous H, Fenel D, Gallet B, Lethier M, Bolla JM, Pagès JM, Vivaudou M, Weik M, Winterhalter M, Colletier JP
Proc Natl Acad Sci U S A 115 E2220-E2228 (2018)
PMID: 29476011

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 352 amino acids
Theoretical weight: 39.03 KDa
Source organism: Providencia stuartii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: E3U904 (Residues: 23-374; Coverage: 94%)
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

3 bound ligands:
Ligand LDA 72 x LDA
Ligand CA 1 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 108.84Å b: 129Å c: 159.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.285
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria.
Vergalli et al. (2020)
1 more

1 mention without citation

Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities.
El-Khatib et al. (2018)