› 5nxx

X-ray diffraction
2.2Å resolution

Crystal structure of OpuAC from B. subtilis in complex with Arsenobetaine

Released:
DOI: 10.2210/pdb5nxx/pdb
PDB entry 5nxx coloured by chain, front view.
PDB entry 5nxx coloured by chain, side view.
PDB entry 5nxx coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Arsenobetaine: an ecophysiologically important organoarsenical confers cytoprotection against osmotic stress and growth temperature extremes.
Hoffmann T, Warmbold B, Smits SHJ, Tschapek B, Ronzheimer S, Bashir A, Chen C, Rolbetzki A, Pittelkow M, Jebbar M, Seubert A, Schmitt L, Bremer E
Environ Microbiol 20 305-323 (2018)
PMID: 29159878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine-binding protein OpuAC Chains: C, D
Molecule details ›
Chains: C, D
Length: 268 amino acids
Theoretical weight: 29.78 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P46922 (Residues: 26-293; Coverage: 98%)
Gene names: BSU03000, opuAC
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 3Q7 2 x 3Q7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 88.1Å b: 30Å c: 106.1Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.188 0.184 0.282
Expression system: Escherichia coli

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Citations

1 review citation

Interactions with Arsenic: Mechanisms of Toxicity and Cellular Resistance in Eukaryotic Microorganisms.
De Francisco et al. (2021)
17 other articles