› 5ny5

X-ray diffraction
2.5Å resolution

The apo structure of 3,4-dihydroxybenzoic acid decarboxylases from Enterobacter cloacae

Released:
DOI: 10.2210/pdb5ny5/pdb
PDB entry 5ny5 coloured by chain, front view.
PDB entry 5ny5 coloured by chain, side view.
PDB entry 5ny5 coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Regioselective para-Carboxylation of Catechols with a Prenylated Flavin Dependent Decarboxylase.
Payer SE, Marshall SA, Bärland N, Sheng X, Reiter T, Dordic A, Steinkellner G, Wuensch C, Kaltwasser S, Fisher K, Rigby SEJ, Macheroux P, Vonck J, Gruber K, Faber K, Himo F, Leys D, Pavkov-Keller T, Glueck SM
Angew Chem Int Ed Engl 56 13893-13897 (2017)
PMID: 28857436

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,4-dihydroxybenzoate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 495 amino acids
Theoretical weight: 54.12 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: B2DCZ6 (Residues: 1-495; Coverage: 100%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: R32
Unit cell:
a: 209.907Å b: 209.907Å c: 162.179Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.236
Expression system: Escherichia coli

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Citations

1 review citation

Synthetic Enzyme-Catalyzed CO2 Fixation Reactions.
Aleku et al. (2021)
11 other articles

2 mentions without citation

Enzymatic Conversion of CO2: From Natural to Artificial Utilization.
Bierbaumer et al. (2023)
1 more