› 5nyw

X-ray diffraction
2.5Å resolution

Anbu (ancestral beta-subunit) from Yersinia bercovieri

Released:
DOI: 10.2210/pdb5nyw/pdb
PDB entry 5nyw coloured by chain, front view.
PDB entry 5nyw coloured by chain, side view.
PDB entry 5nyw coloured by chain, top view.
Source organism: Yersinia bercovieri
Primary publication:
The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.
Piasecka A, Czapinska H, Vielberg MT, Szczepanowski RH, Kiefersauer R, Reed S, Groll M, Bochtler M
J Mol Biol 430 611-627 (2018)
PMID: 29258816

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasome subunit Chains: 1, 2, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 251 amino acids
Theoretical weight: 28.1 KDa
Source organism: Yersinia bercovieri
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

6 bound ligands:
Ligand SO4 17 x SO4
Ligand EDO 6 x EDO
Ligand CL 17 x CL
Ligand UNK 4 x UNK
Ligand AZI 1 x AZI
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 95.535Å b: 285.371Å c: 179.213Å
α: 90° β: 91.78° γ: 90°
R-values:
R R work R free
0.183 0.183 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

An astonishing wealth of new proteasome homologs.
Fuchs et al. (2021)

1 mention without citation

The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.
Piasecka et al. (2018)