› 5nzn

X-ray diffraction
1.73Å resolution

Complex of H275Y/S247N mutant variant of neuraminidase from H1N1 influenza virus with oseltamivir

Released:
DOI: 10.2210/pdb5nzn/pdb
PDB entry 5nzn coloured by chain, front view.
PDB entry 5nzn coloured by chain, side view.
PDB entry 5nzn coloured by chain, top view.
Source organism: unidentified influenza virus
Primary publication:
Kinetic, Thermodynamic, and Structural Analysis of Drug Resistance Mutations in Neuraminidase from the 2009 Pandemic Influenza Virus.
Pokorná J, Pachl P, Karlukova E, Hejdánek J, Řezáčová P, Machara A, Hudlický J, Konvalinka J, Kožíšek M
Viruses 10 (2018)
PMID: 29933553

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 42.78 KDa
Source organism: unidentified influenza virus
Expression system: Escherichia coli
UniProt:
  • Canonical: C3W6G3 (Residues: 82-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 8 x CA
Ligand G39 4 x G39
Ligand NAG 8 x NAG
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 85.778Å b: 127.538Å c: 96.773Å
α: 90° β: 93.24° γ: 90°
R-values:
R R work R free
0.229 0.229 0.261
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Combining structure and genomics to understand antimicrobial resistance.
Tunstall et al. (2020)
8 other articles

1 mention without citation

Kinetic, Thermodynamic, and Structural Analysis of Drug Resistance Mutations in Neuraminidase from the 2009 Pandemic Influenza Virus.
Pokorná et al. (2018)