› 5o0t

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF TRANS-MEMBRANE DOMAIN OF Cylindrospermum stagnale NADPH-OXIDASE 5 (NOX5)

Released:
DOI: 10.2210/pdb5o0t/pdb
PDB entry 5o0t coloured by chain, front view.
PDB entry 5o0t coloured by chain, side view.
PDB entry 5o0t coloured by chain, top view.
Source organism: Cylindrospermum stagnale PCC 7417
Primary publication:
Crystal structures and atomic model of NADPH oxidase.
Magnani F, Nenci S, Millana Fananas E, Ceccon M, Romero E, Fraaije MW, Mattevi A
Proc Natl Acad Sci U S A 114 6764-6769 (2017)
PMID: 28607049

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125714 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ferric reductase Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.47 KDa
Source organism: Cylindrospermum stagnale PCC 7417
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K9WT99 (Residues: 207-412; Coverage: 30%)
Gene name: Cylst_1289
Sequence domains: Ferric reductase like transmembrane component

Ligands and Environments

6 bound ligands:
Ligand SO4 3 x SO4
Ligand HEM 2 x HEM
Ligand LFA 2 x LFA
Ligand D10 3 x D10
Ligand GOL 2 x GOL
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 52.371Å b: 74.57Å c: 85.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

NADPH oxidases and oxidase crosstalk in cardiovascular diseases: novel therapeutic targets.
Zhang et al. (2020)
19 more

15 mentions without citation

Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero et al. (2019)
14 more