› 5o23

X-ray diffraction
2.25Å resolution

Crystal structure of WNK3 kinase domain in a monophosphorylated apo state

Released:
DOI: 10.2210/pdb5o23/pdb
PDB entry 5o23 coloured by chain, front view.
PDB entry 5o23 coloured by chain, side view.
PDB entry 5o23 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pinkas DM, Bufton JC, Kupinska K, Wang D, Sethi R, Newman JA, Chalk R, Berridge G, Burgess-Brown NA, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Bullock A, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase WNK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BYP7 (Residues: 132-414; Coverage: 16%)
Gene names: KIAA1566, PRKWNK3, WNK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand EDO 28 x EDO
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 124.02Å b: 124.02Å c: 107.37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.239
Expression system: Spodoptera frugiperda

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