› 5o26

X-ray diffraction
2.38Å resolution

Crystal structure of WNK3 kinase domain in a diphosphorylated state and in complex with AMP-PNP/Mg2+

Released:
DOI: 10.2210/pdb5o26/pdb
PDB entry 5o26 coloured by chain, front view.
PDB entry 5o26 coloured by chain, side view.
PDB entry 5o26 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pinkas DM, Bufton JC, Newman JA, Kopec J, Borkowska O, Chalk R, Burgess-Brown NA, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Bullock A

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase WNK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BYP7 (Residues: 133-414; Coverage: 16%)
Gene names: KIAA1566, PRKWNK3, WNK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 4 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 36.163Å b: 56.4Å c: 71.236Å
α: 79.79° β: 80.73° γ: 90°
R-values:
R R work R free
0.231 0.228 0.278
Expression system: Spodoptera frugiperda

Quick links