› 5o2x

X-ray diffraction
0.95Å resolution

Extended catalytic domain of H. jecorina LPMO9A a.k.a EG4

Released:
DOI: 10.2210/pdb5o2x/pdb
Model geometry
Fit model/data
PDB entry 5o2x coloured by chain, front view.
PDB entry 5o2x coloured by chain, side view.
PDB entry 5o2x coloured by chain, top view.
Source organism: Trichoderma reesei QM6a
Primary publication:
High-resolution structure of a lytic polysaccharide monooxygenase from Hypocrea jecorina reveals a predicted linker as an integral part of the catalytic domain.
Hansson H, Karkehabadi S, Mikkelsen N, Douglas NR, Kim S, Lam A, Kaper T, Kelemen B, Meier KK, Jones SM, Solomon EI, Sandgren M
J Biol Chem 292 19099-19109 (2017)
PMID: 28900033

Function and Biology Details

Reaction catalysed: 
((1->4)-beta-D-glucosyl)(n+m) + reduced acceptor + O(2) = 4-dehydro-beta-D-glucosyl-((1->4)-beta-D-glucosyl)(n-1) + ((1->4)-beta-D-glucosyl)(m) + acceptor + H(2)O
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AA9 family lytic polysaccharide monooxygenase cel61A Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 26.13 KDa
Source organism: Trichoderma reesei QM6a
Expression system: Trichoderma reesei RUT C-30
UniProt:
  • Canonical: G0R6T8 (Residues: 23-269; Coverage: 77%)
Gene names: LPMO9A, TRIREDRAFT_73643, cel61a
Sequence domains: Auxiliary Activity family 9 (formerly GH61)
Structure domains: Coagulation Factor XIII; Chain A, domain 1

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand MAN 15 x MAN
Ligand CU 1 x CU
Ligand SO4 3 x SO4
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 42.82Å b: 61.55Å c: 47.78Å
α: 90° β: 112.03° γ: 90°
R-values:
R R work R free
0.116 0.115 0.127
Expression system: Trichoderma reesei RUT C-30

Quick links

Citations

4 review citations

Distinct Substrate Specificities and Electron-Donating Systems of Fungal Lytic Polysaccharide Monooxygenases.
Frommhagen et al. (2018)
3 more

9 mentions without citation

Oxygen Activation by Cu LPMOs in Recalcitrant Carbohydrate Polysaccharide Conversion to Monomer Sugars.
Meier et al. (2018)
8 more