› 5o33

X-ray diffraction
1.64Å resolution

A structure of the GEF Kalirin DH1 domain in complex with the small GTPase Rac1

Released:
DOI: 10.2210/pdb5o33/pdb
PDB entry 5o33 coloured by chain, front view.
PDB entry 5o33 coloured by chain, side view.
PDB entry 5o33 coloured by chain, top view.
Source organisms:
Entry authors: Gray J, Krojer T, Talon R, Fairhead M, Bountra C, Arrowsmith CH, Edwards A, Brennan P, von Delft F

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158898 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Kalirin Chain: B
Molecule details ›
Chain: B
Length: 180 amino acids
Theoretical weight: 21.06 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P97924 (Residues: 1253-1432; Coverage: 6%)
Gene names: Duo, Hapip, Kalrn
Sequence domains: RhoGEF domain
Structure domains: Dbl homology (DH) domain

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6522
Unit cell:
a: 63.255Å b: 63.255Å c: 346.704Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.207 0.24
Expression system: Escherichia coli

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