› 5o65

X-ray diffraction
2.5Å resolution

Crystal Structure of the Pseudomonas functional amyloid secretion protein FapF

Released:
DOI: 10.2210/pdb5o65/pdb
PDB entry 5o65 coloured by chain, front view.
PDB entry 5o65 coloured by chain, side view.
PDB entry 5o65 coloured by chain, top view.
Source organism: Pseudomonas sp. UK4
Primary publication:
A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis.
Rouse SL, Hawthorne WJ, Berry JL, Chorev DS, Ionescu SA, Lambert S, Stylianou F, Ewert W, Mackie U, Morgan RML, Otzen D, Herbst FA, Nielsen PH, Dueholm M, Bayley H, Robinson CV, Hare S, Matthews S
Nat Commun 8 263 (2017)
PMID: 28811582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FapF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 334 amino acids
Theoretical weight: 36.73 KDa
Source organism: Pseudomonas sp. UK4
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IN73 (Residues: 107-430; Coverage: 80%)
Gene names: PSUK4_00060, fapF

Ligands and Environments

3 bound ligands:
Ligand LDA 7 x LDA
Ligand C8E 6 x C8E
Ligand NA 3 x NA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 143.26Å b: 124.731Å c: 80.359Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.223 0.222 0.259
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Functional Amyloids.
Otzen et al. (2019)
7 more

2 mentions without citation

Functional Bacterial Amyloids: Understanding Fibrillation, Regulating Biofilm Fibril Formation and Organizing Surface Assemblies.
Sønderby et al. (2022)
1 more