› 5o67

X-ray diffraction
2.84Å resolution

Crystal structure of the FapF polypeptide transporter - F103A mutant

Released:
DOI: 10.2210/pdb5o67/pdb
PDB entry 5o67 coloured by chain, front view.
PDB entry 5o67 coloured by chain, side view.
PDB entry 5o67 coloured by chain, top view.
Source organism: Pseudomonas sp. UK4
Primary publication:
A new class of hybrid secretion system is employed in Pseudomonas amyloid biogenesis.
Rouse SL, Hawthorne WJ, Berry JL, Chorev DS, Ionescu SA, Lambert S, Stylianou F, Ewert W, Mackie U, Morgan RML, Otzen D, Herbst FA, Nielsen PH, Dueholm M, Bayley H, Robinson CV, Hare S, Matthews S
Nat Commun 8 263 (2017)
PMID: 28811582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111288 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
FapF Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 36.39 KDa
Source organism: Pseudomonas sp. UK4
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IN73 (Residues: 107-430; Coverage: 80%)
Gene names: PSUK4_00060, fapF
FapF Chains: B, C
Molecule details ›
Chains: B, C
Length: 334 amino acids
Theoretical weight: 36.38 KDa
Source organism: Pseudomonas sp. UK4
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IN73 (Residues: 107-430; Coverage: 80%)
Gene names: PSUK4_00060, fapF

Ligands and Environments

2 bound ligands:
Ligand LDA 5 x LDA
Ligand C8E 3 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 147.753Å b: 125.816Å c: 81.908Å
α: 90° β: 96.68° γ: 90°
R-values:
R R work R free
0.235 0.232 0.295
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Half a century of amyloids: past, present and future.
Ke et al. (2020)
7 more

4 mentions without citation

Functional Amyloids Are the Rule Rather Than the Exception in Cellular Biology.
Balistreri et al. (2020)
3 more