Function and Biology Details
Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
homo tetramer (preferred)
Assembly name:
Ubiquitin carboxyl-terminal hydrolase 25 (preferred)
PDBe Complex ID:
PDB-CPX-193759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase 25
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 82.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 716 amino acids
Theoretical weight: 82.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9UHP3 (Residues: 1-714; Coverage: 68%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALBA BEAMLINE XALOC
Spacegroup:
I422
Expression system: Escherichia coli
