› 5o7t

X-ray diffraction
1.8Å resolution

Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair

Released:
DOI: 10.2210/pdb5o7t/pdb
PDB entry 5o7t coloured by chain, front view.
PDB entry 5o7t coloured by chain, side view.
PDB entry 5o7t coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
Betz K, Nilforoushan A, Wyss LA, Diederichs K, Sturla SJ, Marx A
Chem Commun (Camb) 53 12704-12707 (2017)
PMID: 29136072

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119087 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I, thermostable Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 60.93 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19821 (Residues: 293-832; Coverage: 65%)
Gene names: pol1, polA
Sequence domains:
Structure domains:
DNA primer Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA template Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MG 3 x MG
Ligand DCP 1 x DCP
Ligand GOL 1 x GOL
1 modified residue:
Ligand DDG 1 x DDG

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 109.476Å b: 109.476Å c: 91.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Modified nucleic acids: replication, evolution, and next-generation therapeutics.
Duffy et al. (2020)
4 other articles

1 mention without citation

Sequence-Specific Quantitation of Mutagenic DNA Damage via Polymerase Amplification with an Artificial Nucleotide.
Aloisi et al. (2020)