› 5o7x

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF S. CEREVISIAE CORE FACTOR AT 3.2A RESOLUTION

Released:
DOI: 10.2210/pdb5o7x/pdb
PDB entry 5o7x coloured by chain, front view.
PDB entry 5o7x coloured by chain, side view.
PDB entry 5o7x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural Basis of RNA Polymerase I Transcription Initiation.
Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P
Cell 169 120-131.e22 (2017)
PMID: 28340337

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-152453 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RNA polymerase I-specific transcription initiation factor RRN6 Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 894 amino acids
Theoretical weight: 102.16 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32786 (Residues: 1-894; Coverage: 100%)
Gene names: RRN6, YBL014C, YBL0311, YBL0312
Sequence domains:
RNA polymerase I-specific transcription initiation factor RRN7 Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 514 amino acids
Theoretical weight: 60.44 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40992 (Residues: 1-514; Coverage: 100%)
Gene names: J1273, RRN7, YJL025W
Sequence domains:
RNA polymerase I-specific transcription initiation factor RRN11 Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 507 amino acids
Theoretical weight: 59.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04712 (Residues: 1-507; Coverage: 100%)
Gene names: RRN11, YM9827.09C, YML043C
Sequence domains: RNA polymerase I-specific transcription initiation factor Rrn11

Ligands and Environments

2 bound ligands:
Ligand SO4 30 x SO4
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 109.07Å b: 109.14Å c: 385.64Å
α: 90.02° β: 90.01° γ: 59.98°
R-values:
R R work R free
0.255 0.254 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Organization and regulation of gene transcription.
Cramer P. (2019)
19 more

2 mentions without citation

Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting.
Pilsl et al. (2020)
1 more