› 5o7y

X-ray diffraction
1.97Å resolution

Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum in complex with succinate

Released:
DOI: 10.2210/pdb5o7y/pdb
PDB entry 5o7y coloured by chain, front view.
PDB entry 5o7y coloured by chain, side view.
PDB entry 5o7y coloured by chain, top view.
Source organism: Papaver somniferum
Primary publication:
Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway.
Kluza A, Niedzialkowska E, Kurpiewska K, Wojdyla Z, Quesne M, Kot E, Porebski PJ, Borowski T
J Struct Biol 202 229-235 (2018)
PMID: 29408320

Function and Biology Details

Reaction catalysed: 
Thebaine + 2-oxoglutarate + O(2) = neopinone + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thebaine 6-O-demethylase Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 40.94 KDa
Source organism: Papaver somniferum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4N500 (Residues: 1-364; Coverage: 100%)
Gene names: C5167_018133, DIOX1, T6ODM
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NI 1 x NI
Ligand SIN 1 x SIN
Ligand EDO 17 x EDO
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: Agilent SuperNova
Spacegroup: P212121
Unit cell:
a: 47.231Å b: 87.028Å c: 94.171Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Unleashing the Synthetic Power of Plant Oxygenases: From Mechanism to Application.
Mitchell et al. (2019)
3 more

3 mentions without citation

Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway.
Kluza et al. (2018)
2 more