› 5o8y

X-ray diffraction
2.3Å resolution

Conformational dynamism for DNA interaction in Salmonella typhimurium RcsB response regulator.

Released:
DOI: 10.2210/pdb5o8y/pdb
PDB entry 5o8y coloured by chain, front view.
PDB entry 5o8y coloured by chain, side view.
PDB entry 5o8y coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Conformational dynamism for DNA interaction in the Salmonella RcsB response regulator.
Casino P, Miguel-Romero L, Huesa J, García P, García-Del Portillo F, Marina A
Nucleic Acids Res 46 456-472 (2018)
PMID: 29186528

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein RcsB Chains: A, C, D, F, G, H
Molecule details ›
Chains: A, C, D, F, G, H
Length: 216 amino acids
Theoretical weight: 23.74 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P58663 (Residues: 1-216; Coverage: 100%)
Gene names: STM2270, rcsB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand PE4 2 x PE4
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P41212
Unit cell:
a: 108.83Å b: 108.83Å c: 306.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Complex Rcs Regulatory Cascade.
Wall et al. (2018)
9 other articles

2 mentions without citation

Conformational dynamism for DNA interaction in the Salmonella RcsB response regulator.
Casino et al. (2018)
1 more