› 5o8z

X-ray diffraction
2.1Å resolution

Conformational dynamism for DNA interaction in Salmonella typhimurium RcsB response regulator.

Released:
DOI: 10.2210/pdb5o8z/pdb
PDB entry 5o8z coloured by chain, front view.
PDB entry 5o8z coloured by chain, side view.
PDB entry 5o8z coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Conformational dynamism for DNA interaction in the Salmonella RcsB response regulator.
Casino P, Miguel-Romero L, Huesa J, García P, García-Del Portillo F, Marina A
Nucleic Acids Res 46 456-472 (2018)
PMID: 29186528

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein RcsB Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.74 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P58663 (Residues: 1-216; Coverage: 100%)
Gene names: STM2270, rcsB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BEF 2 x BEF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P1
Unit cell:
a: 37.392Å b: 54.266Å c: 54.986Å
α: 62.6° β: 81.61° γ: 79.66°
R-values:
R R work R free
0.215 0.213 0.265
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Complex Rcs Regulatory Cascade.
Wall et al. (2018)
9 other articles

2 mentions without citation

Conformational dynamism for DNA interaction in the Salmonella RcsB response regulator.
Casino et al. (2018)
1 more