› 5o99

X-ray diffraction
0.87Å resolution

Unconventional SH3 domain from the postsynaptic density scaffold protein Shank3

Released:
DOI: 10.2210/pdb5o99/pdb
PDB entry 5o99 coloured by chain, front view.
PDB entry 5o99 coloured by chain, side view.
PDB entry 5o99 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of an unconventional SH3 domain from the postsynaptic density protein Shank3 at ultrahigh resolution.
Ponna SK, Myllykoski M, Boeckers TM, Kursula P
Biochem Biophys Res Commun 490 806-812 (2017)
PMID: 28647360

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SH3 and multiple ankyrin repeat domains protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 6.44 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JLU4 (Residues: 470-528; Coverage: 3%)
Gene names: Prosap2, Shank3
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand BTB 1 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 29.74Å b: 52.91Å c: 31.66Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.112 0.097 0.117
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Abnormalities in interactions of Rho GTPases with scaffolding proteins contribute to neurodevelopmental disorders.
Reichova et al. (2018)
1 more

4 mentions without citation

Autism-associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses.
Bucher et al. (2021)
3 more