› 5occ

X-ray diffraction
2.5Å resolution

Crystal structure of CD32b (Fc Gamma Receptor IIb) in complex with Human IgG1 Fab fragment (6G08)

Released:
DOI: 10.2210/pdb5occ/pdb
PDB entry 5occ coloured by chain, front view.
PDB entry 5occ coloured by chain, side view.
PDB entry 5occ coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering.
Sutton EJ, Bradshaw RT, Orr CM, Frendéus B, Larsson G, Teige I, Cragg MS, Tews I, Essex JW
Biophys J 115 289-299 (2018)
PMID: 30021105

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211054 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Low affinity immunoglobulin gamma Fc region receptor II-b Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 19.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31994 (Residues: 43-218; Coverage: 66%)
Gene names: CD32, FCG2, FCGR2B, IGFR2
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins
6G08 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
6G08 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 217 amino acids
Theoretical weight: 22.82 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand GOL 2 x GOL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 70.23Å b: 75.02Å c: 134.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.192 0.261
Expression system: Homo sapiens

Quick links

Citations

1 review citation

The Structural and Functional Diversity of Intrinsically Disordered Regions in Transmembrane Proteins.
Appadurai et al. (2019)
1 other article

2 mentions without citation

Evaluating Anti-CD32b F(ab) Conformation Using Molecular Dynamics and Small-Angle X-Ray Scattering.
Sutton et al. (2018)
1 more