› 5od6

X-ray diffraction
2Å resolution

Crystal structure of Smad3-MH1 bound to the GGCGC site.

Released:
DOI: 10.2210/pdb5od6/pdb
PDB entry 5od6 coloured by chain, front view.
PDB entry 5od6 coloured by chain, side view.
PDB entry 5od6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors.
Martin-Malpartida P, Batet M, Kaczmarska Z, Freier R, Gomes T, Aragón E, Zou Y, Wang Q, Xi Q, Ruiz L, Vea A, Márquez JA, Massagué J, Macias MJ
Nat Commun 8 2070 (2017)
PMID: 29234012

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119091 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 15.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P84022 (Residues: 11-135; Coverage: 29%)
Gene names: MADH3, SMAD3
Sequence domains: MH1 domain
Structure domains: SMAD MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*GP*CP*GP*CP*GP*CP*CP*TP*GP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I41
Unit cell:
a: 105.2Å b: 105.2Å c: 73.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.235
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Contextual determinants of TGFβ action in development, immunity and cancer.
David et al. (2018)
15 more

4 mentions without citation

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors.
Martin-Malpartida et al. (2017)
3 more