› 5oes

X-ray diffraction
2.48Å resolution

The structure of a glutathione synthetase (StGSS1) from Solanum tuberosum in ADP and y-EC bound closed conformation.

Released:
DOI: 10.2210/pdb5oes/pdb
PDB entry 5oes coloured by chain, front view.
PDB entry 5oes coloured by chain, side view.
PDB entry 5oes coloured by chain, top view.
Source organism: Solanum tuberosum
Primary publication:
Effector gene birth in plant parasitic nematodes: Neofunctionalization of a housekeeping glutathione synthetase gene.
Lilley CJ, Maqbool A, Wu D, Yusup HB, Jones LM, Birch PRJ, Banfield MJ, Urwin PE, Eves-van den Akker S
PLoS Genet 14 e1007310 (2018)
PMID: 29641602

Function and Biology Details

Reaction catalysed: 
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione synthetase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 483 amino acids
Theoretical weight: 54.4 KDa
Source organism: Solanum tuberosum
Expression system: Escherichia coli
UniProt:
  • Canonical: M1CSC4 (Residues: 1-483; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand 3GC 6 x 3GC
2 bound ligands:
Ligand ADP 6 x ADP
Ligand MG 10 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P41212
Unit cell:
a: 154.39Å b: 154.39Å c: 344.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

All Roads Lead to Susceptibility: The Many Modes of Action of Fungal and Oomycete Intracellular Effectors.
He et al. (2020)
2 more

2 mentions without citation

Effector gene birth in plant parasitic nematodes: Neofunctionalization of a housekeeping glutathione synthetase gene.
Lilley et al. (2018)
1 more