› 5oeu

X-ray diffraction
2.57Å resolution

The structure of a glutathione synthetase like-effector (GSS22) from Globodera pallida in ADP-bound closed conformation.

Released:
DOI: 10.2210/pdb5oeu/pdb
PDB entry 5oeu coloured by chain, front view.
PDB entry 5oeu coloured by chain, side view.
PDB entry 5oeu coloured by chain, top view.
Source organism: Globodera pallida
Primary publication:
Effector gene birth in plant parasitic nematodes: Neofunctionalization of a housekeeping glutathione synthetase gene.
Lilley CJ, Maqbool A, Wu D, Yusup HB, Jones LM, Birch PRJ, Banfield MJ, Urwin PE, Eves-van den Akker S
PLoS Genet 14 e1007310 (2018)
PMID: 29641602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione synthetase Chains: A, C
Molecule details ›
Chains: A, C
Length: 510 amino acids
Theoretical weight: 57.43 KDa
Source organism: Globodera pallida
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A183CD52 (Residues: 1-9, 10-97, 98-300; Coverage: 100%)
Sequence domains: Eukaryotic glutathione synthase, ATP binding domain

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 80.27Å b: 121.079Å c: 128.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

All Roads Lead to Susceptibility: The Many Modes of Action of Fungal and Oomycete Intracellular Effectors.
He et al. (2020)
2 more

1 mention without citation

Effector gene birth in plant parasitic nematodes: Neofunctionalization of a housekeeping glutathione synthetase gene.
Lilley et al. (2018)