› 5ohv

X-ray diffraction
2.8Å resolution

K33-specific affimer bound to K33 diUb

Released:
DOI: 10.2210/pdb5ohv/pdb
PDB entry 5ohv coloured by chain, front view.
PDB entry 5ohv coloured by chain, side view.
PDB entry 5ohv coloured by chain, top view.
Source organisms:
Primary publication:
Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling.
Michel MA, Swatek KN, Hospenthal MK, Komander D
Mol Cell 68 233-246.e5 (2017)
PMID: 28943312

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143431 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: A, C
Molecule details ›
Chains: A, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
K33-specific affimer Chains: B, D
Molecule details ›
Chains: B, D
Length: 116 amino acids
Theoretical weight: 13.24 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: R3
Unit cell:
a: 120.288Å b: 120.288Å c: 69.973Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.224
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Building and decoding ubiquitin chains for mitophagy.
Harper et al. (2018)
38 more

1 mention without citation

Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling.
Michel et al. (2017)