› 5oj1

X-ray diffraction
2.85Å resolution

Penicillin Binding Protein 2x (PBP2x) from S.pneumoniae in complex with Oxacillin and a tetrasaccharide

Released:
DOI: 10.2210/pdb5oj1/pdb
PDB entry 5oj1 coloured by chain, front view.
PDB entry 5oj1 coloured by chain, side view.
PDB entry 5oj1 coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
Allostery, Recognition of Nascent Peptidoglycan, and Cross-linking of the Cell Wall by the Essential Penicillin-Binding Protein 2x of Streptococcus pneumoniae.
Bernardo-García N, Mahasenan KV, Batuecas MT, Lee M, Hesek D, Petráčková D, Doubravová L, Branny P, Mobashery S, Hermoso JA
ACS Chem Biol 13 694-702 (2018)
PMID: 29357220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 2X Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 76.81 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: P59676 (Residues: 49-750; Coverage: 94%)
Gene names: pbpX, spr0304
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand 1S6 1 x 1S6
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P3121
Unit cell:
a: 100.839Å b: 100.839Å c: 189.354Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.167 0.229
Expression system: Escherichia coli

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Citations

1 review citation

Constructing and deconstructing the bacterial cell wall.
Fisher et al. (2020)
15 other articles