› 5ojy

X-ray diffraction
1.85Å resolution

Co-complex structure of regulator protein 2 (PamR2) with pamamycin 607 from Streptomyces alboniger

Released:
DOI: 10.2210/pdb5ojy/pdb
PDB entry 5ojy coloured by chain, front view.
PDB entry 5ojy coloured by chain, side view.
PDB entry 5ojy coloured by chain, top view.
Source organism: Streptomyces alboniger
Primary publication:
Design, development and application of whole-cell based antibiotic-specific biosensor.
Rebets Y, Schmelz S, Gromyko O, Tistechok S, Petzke L, Scrima A, Luzhetskyy A
Metab Eng 47 263-270 (2018)
PMID: 29609044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101113 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.28 KDa
Source organism: Streptomyces alboniger
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0D3RJT7 (Residues: 2-245; Coverage: 100%)
Gene names: CP975_33745, SALB_00464, pamR2
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand CIT 1 x CIT
Ligand 9XB 1 x 9XB
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6222
Unit cell:
a: 107.85Å b: 107.85Å c: 119.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.196
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Activation of microbial secondary metabolic pathways: Avenues and challenges.
Baral et al. (2018)
5 more