› 5okl

X-ray diffraction
2.09Å resolution

Human afamin monoclinic crystal form

Released:
DOI: 10.2210/pdb5okl/pdb
PDB entry 5okl coloured by chain, front view.
PDB entry 5okl coloured by chain, side view.
PDB entry 5okl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Evidence for a Role of the Multi-functional Human Glycoprotein Afamin in Wnt Transport.
Naschberger A, Orry A, Lechner S, Bowler MW, Nurizzo D, Novokmet M, Keller MA, Oemer G, Seppi D, Haslbeck M, Pansi K, Dieplinger H, Rupp B
Structure 25 1907-1915.e5 (2017)
PMID: 29153507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155116 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Afamin Chains: A, B
Molecule details ›
Chains: A, B
Length: 586 amino acids
Theoretical weight: 67.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P43652 (Residues: 22-599; Coverage: 100%)
Gene names: AFM, ALB2, ALBA
Sequence domains: Serum albumin family

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CL 3 x CL
Ligand NAG 1 x NAG
Ligand PAM 1 x PAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21
Unit cell:
a: 50.915Å b: 112.872Å c: 109.228Å
α: 90° β: 93.39° γ: 90°
R-values:
R R work R free
0.201 0.199 0.237
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

Mechanisms of intercellular Wnt transport.
Routledge et al. (2019)
4 more

2 mentions without citation

Fatty acid recognition in the Frizzled receptor family.
Nile et al. (2019)
1 more