› 5ol2

X-ray diffraction
3.1Å resolution

The electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile

Released:
DOI: 10.2210/pdb5ol2/pdb
PDB entry 5ol2 coloured by chain, front view.
PDB entry 5ol2 coloured by chain, side view.
PDB entry 5ol2 coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile.
Demmer JK, Pal Chowdhury N, Selmer T, Ermler U, Buckel W
Nat Commun 8 1577 (2017)
PMID: 29146947

Function and Biology Details

Reaction catalysed: 
A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-102870 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Electron transfer flavoprotein alpha/beta-subunit N-terminal domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 331 amino acids
Theoretical weight: 35.59 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A125V455 (Residues: 1-331; Coverage: 99%)
Gene names: CDIF1296T_01151, etfA2
Sequence domains:
Electron transfer flavoprotein small subunit Chains: B, E
Molecule details ›
Chains: B, E
Length: 260 amino acids
Theoretical weight: 28.35 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A125V3V6 (Residues: 1-260; Coverage: 100%)
Gene names: CDIF1296T_01150, etfB2
Sequence domains: Electron transfer flavoprotein domain
Structure domains: HUPs
Butyryl-CoA dehydrogenase Chains: C, F
Molecule details ›
Chains: C, F
Length: 378 amino acids
Theoretical weight: 41.35 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A125V4E7 (Residues: 1-378; Coverage: 100%)
Gene names: CDIF1296T_01149, bcd2
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 6 x FAD
2 bound ligands:
Ligand CA 5 x CA
Ligand COS 2 x COS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I4122
Unit cell:
a: 177.06Å b: 177.06Å c: 493.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.26
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review.
Buckel et al. (2018)
9 more

6 mentions without citation

The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile.
Demmer et al. (2017)
5 more