› 5ol8

X-ray diffraction
1.9Å resolution

Structure of human mitochondrial transcription elongation factor (TEFM) C-terminal domain

Released:
DOI: 10.2210/pdb5ol8/pdb
PDB entry 5ol8 coloured by chain, front view.
PDB entry 5ol8 coloured by chain, side view.
PDB entry 5ol8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of Transcription Anti-termination in Human Mitochondria.
Hillen HS, Parshin AV, Agaronyan K, Morozov YI, Graber JJ, Chernev A, Schwinghammer K, Urlaub H, Anikin M, Cramer P, Temiakov D
Cell 171 1082-1093.e13 (2017)
PMID: 29033127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 319 amino acids
Theoretical weight: 37.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QE5 (Residues: 51-360; Coverage: 86%)
Gene names: C17orf42, TEFM
Sequence domains: Helix-hairpin-helix motif

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 98.48Å b: 112.54Å c: 88.84Å
α: 90° β: 110.13° γ: 90°
R-values:
R R work R free
0.197 0.195 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.
Basu et al. (2020)
14 more

2 mentions without citation

Structural basis of mitochondrial transcription.
Hillen et al. (2018)
1 more