› 5ol9

X-ray diffraction
1.3Å resolution

Structure of human mitochondrial transcription elongation factor (TEFM) N-terminal domain

Released:
DOI: 10.2210/pdb5ol9/pdb
PDB entry 5ol9 coloured by chain, front view.
PDB entry 5ol9 coloured by chain, side view.
PDB entry 5ol9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of Transcription Anti-termination in Human Mitochondria.
Hillen HS, Parshin AV, Agaronyan K, Morozov YI, Graber JJ, Chernev A, Schwinghammer K, Urlaub H, Anikin M, Cramer P, Temiakov D
Cell 171 1082-1093.e13 (2017)
PMID: 29033127

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188698 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QE5 (Residues: 36-134; Coverage: 28%)
Gene names: C17orf42, TEFM
Sequence domains: Helix-hairpin-helix motif

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P43212
Unit cell:
a: 47.67Å b: 47.67Å c: 93.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.
Basu et al. (2020)
14 more

3 mentions without citation

Structural basis of mitochondrial transcription.
Hillen et al. (2018)
2 more