Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- DNA-directed RNA polymerase, phage-type
- DNA-directed RNA polymerase, N-terminal
- Ribonuclease H-like superfamily
- Ribonuclease H superfamily
- Transcription elongation factor, mitochondrial
- DNA-directed RNA polymerase, C-terminal domain, phage-type
- DNA-directed RNA polymerase, N-terminal domain superfamily
- DNA/RNA polymerase superfamily
1 more domain
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119100 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Transcription elongation factor, mitochondrial
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 27.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 234 amino acids
Theoretical weight: 27.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q96QE5 (Residues: 136-360; Coverage: 63%)
DNA-directed RNA polymerase, mitochondrial
Molecule details ›
Chains: E, F
Length: 1088 amino acids
Theoretical weight: 123.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 1088 amino acids
Theoretical weight: 123.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O00411 (Residues: 151-1230; Coverage: 88%)
Sequence domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X10SA
Spacegroup:
C2
Expression system: Escherichia coli BL21(DE3)
