› 5omr

X-ray diffraction
1.68Å resolution

Crystal structure of Amycolatopsis cytochrome P450 GcoA in complex with vanillin.

Released:
DOI: 10.2210/pdb5omr/pdb
PDB entry 5omr coloured by chain, front view.
PDB entry 5omr coloured by chain, side view.
PDB entry 5omr coloured by chain, top view.
Source organism: Amycolatopsis sp. ATCC 39116
Primary publication:
A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.
Mallinson SJB, Machovina MM, Silveira RL, Garcia-Borràs M, Gallup N, Johnson CW, Allen MD, Skaf MS, Crowley MF, Neidle EL, Houk KN, Beckham GT, DuBois JL, McGeehan JE
Nat Commun 9 2487 (2018)
PMID: 29950589

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 45.45 KDa
Source organism: Amycolatopsis sp. ATCC 39116
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3B6UEE9 (Residues: 1-409; Coverage: 100%)
Sequence domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand V55 1 x V55
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 104.11Å b: 104.11Å c: 115.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.17
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products.
Xu et al. (2019)
3 more

2 mentions without citation

A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.
Mallinson et al. (2018)
1 more