› 5ond

X-ray diffraction
2.1Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119105 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription antitermination protein RfaH Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFW0 (Residues: 1-162; Coverage: 100%)
Gene names: JW3818, b3842, hlyT, rfaH, sfrB
Sequence domains: Transcription termination factor nusG
DNA (5'-D(*GP*CP*GP*GP*TP*AP*GP*TP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 nucleotides
Theoretical weight: 2.77 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 36.31Å b: 43.19Å c: 61.86Å
α: 80.45° β: 75.48° γ: 75.39°
R-values:
R R work R free
0.189 0.186 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Mechanisms of Transcriptional Pausing in Bacteria.
Kang et al. (2019)
7 more

14 mentions without citation

NusG, an Ancient Yet Rapidly Evolving Transcription Factor.
Wang et al. (2020)
13 more