› 5onu

X-ray diffraction
2.22Å resolution

Trimeric OmpU structure

Released:
DOI: 10.2210/pdb5onu/pdb
PDB entry 5onu coloured by chain, front view.
PDB entry 5onu coloured by chain, side view.
PDB entry 5onu coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of the outer membrane protein OmpU from Vibrio cholerae at 2.2 Å resolution.
Li H, Zhang W, Dong C
Acta Crystallogr D Struct Biol 74 21-29 (2018)
PMID: 29372896

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein U Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 356 amino acids
Theoretical weight: 38.52 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpB1_S
UniProt:
  • Canonical: P0C6Q6 (Residues: 1-341; Coverage: 100%)
Gene names: VC_0633, ompU
Sequence domains: Gram-negative porin

Ligands and Environments

3 bound ligands:
Ligand C8E 3 x C8E
Ligand GOL 6 x GOL
Ligand LDA 10 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 129.88Å b: 153.47Å c: 81.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.236
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpB1_S

Quick links

Citations

2 review citations

Steering Phages to Combat Bacterial Pathogens.
Gurney et al. (2020)
1 more

4 mentions without citation

New tools in MolProbity validation: CaBLAM for CryoEM backbone, UnDowser to rethink "waters," and NGL Viewer to recapture online 3D graphics.
Prisant et al. (2020)
3 more