› 5oo7

X-ray diffraction
1.84Å resolution

The ENTH domain from epsin-2 in complex with phosphatidylinositol 4,5-bisphosphate (PIP2)

Released:
DOI: 10.2210/pdb5oo7/pdb
PDB entry 5oo7 coloured by chain, front view.
PDB entry 5oo7 coloured by chain, side view.
PDB entry 5oo7 coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Epsin and Sla2 form assemblies through phospholipid interfaces.
Garcia-Alai MM, Heidemann J, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R
Nat Commun 9 328 (2018)
PMID: 29362354

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SLA2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.68 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S106 (Residues: 6-262; Coverage: 25%)
Gene name: CTHT_0011900
Sequence domains: ANTH domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 47.513Å b: 106.28Å c: 51.291Å
α: 90° β: 98.15° γ: 90°
R-values:
R R work R free
0.206 0.204 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Novel roles of phosphoinositides in signaling, lipid transport, and disease.
Hammond et al. (2020)
2 more

4 mentions without citation

Epsin and Sla2 form assemblies through phospholipid interfaces.
Garcia-Alai et al. (2018)
3 more