› 5ood

Electron Microscopy
3.7Å resolution

Cryo-EM structure of jasplakinolide-stabilized F-actin in complex with ADP-Pi

Released:
DOI: 10.2210/pdb5ood/pdb
PDB entry 5ood coloured by chain, front view.
PDB entry 5ood coloured by chain, side view.
PDB entry 5ood coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structural transitions of F-actin upon ATP hydrolysis at near-atomic resolution revealed by cryo-EM.
Merino F, Pospich S, Funk J, Wagner T, Küllmer F, Arndt HD, Bieling P, Raunser S
Nat Struct Mol Biol 25 528-537 (2018)
PMID: 29867215
Related structures: EMD-3837

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin

Ligands and Environments

4 bound ligands:
Ligand ADP 5 x ADP
Ligand PO4 5 x PO4
Ligand 9ZK 5 x 9ZK
Ligand MG 5 x MG
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-3837

Quick links

Citations

9 review citations

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
8 more

3 mentions without citation

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Pospich et al. (2021)
2 more