› 5or5

Solution NMR

NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA

Released:
DOI: 10.2210/pdb5or5/pdb
PDB entry 5or5 coloured by chain, ensemble of 13 models, front view.
PDB entry 5or5 coloured by chain, ensemble of 13 models, side view.
PDB entry 5or5 coloured by chain, ensemble of 13 models, top view.
Source organisms:
Primary publication:
Engineered promoter selectivity of an ECF sigma factor
Campagne S, Vorholt JA, Allain FH
To be published

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119114 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
ECF RNA polymerase sigma factor SigW; ECF RNA polymerase sigma-E factor Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.99 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGB6 (Residues: 1-64, 79-92; Coverage: 41%)
  • Canonical: Q45585 (Residues: 64-69, 71-77; Coverage: 7%)
Gene names: BSU01730, JW2557, b2573, rpoE, sigE, sigW, ybbL
Sequence domains: Sigma-70 region 2
Structure domains: Rna Polymerase Sigma Factor; Chain: A
DNA (5'-D(*GP*TP*AP*AP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.76 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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