› 5osh

X-ray diffraction
4.3Å resolution

Structure of retromer VPS29-VPS35C subunits complexed with RidL N-terminal domain (1-236)

Released:
DOI: 10.2210/pdb5osh/pdb
PDB entry 5osh coloured by chain, front view.
PDB entry 5osh coloured by chain, side view.
PDB entry 5osh coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.
Romano-Moreno M, Rojas AL, Williamson CD, Gershlick DC, Lucas M, Isupov MN, Bonifacino JS, Machner MP, Hierro A
Proc Natl Acad Sci U S A 114 E11151-E11160 (2017)
PMID: 29229824

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-166066 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vacuolar protein sorting-associated protein 29 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 182 amino acids
Theoretical weight: 20.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBQ0 (Residues: 1-182; Coverage: 100%)
Gene names: DC15, DC7, MDS007, VPS29
Sequence domains: Calcineurin-like phosphoesterase superfamily domain
Vacuolar protein sorting-associated protein 35 Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 299 amino acids
Theoretical weight: 34.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96QK1 (Residues: 482-780; Coverage: 38%)
Gene names: MEM3, TCCCTA00141, VPS35
Sequence domains: Vacuolar protein sorting-associated protein 35
Interaptin Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 223 amino acids
Theoretical weight: 25.63 KDa
Source organism: Legionella pneumophila subsp. pneumophila ATCC 43290
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 90.201Å b: 173.243Å c: 445.775Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.254 0.311
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Pathogenicity and Virulence of Legionella: Intracellular replication and host response.
Chauhan et al. (2021)
8 more

2 mentions without citation

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL.
Romano-Moreno et al. (2017)
1 more