› 5ouz

X-ray diffraction
2.08Å resolution

Metal free structure of Y40F SynFtn

Released:
DOI: 10.2210/pdb5ouz/pdb
PDB entry 5ouz coloured by chain, front view.
PDB entry 5ouz coloured by chain, side view.
PDB entry 5ouz coloured by chain, top view.
Source organism: Synechococcus sp. CC9311
Primary publication:
Reaction of O2 with a diiron protein generates a mixed-valent Fe2+/Fe3+ center and peroxide.
Bradley JM, Svistunenko DA, Pullin J, Hill N, Stuart RK, Palenik B, Wilson MT, Hemmings AM, Moore GR, Le Brun NE
Proc Natl Acad Sci U S A 116 2058-2067 (2019)
PMID: 30659147

Function and Biology Details

Reaction catalysed: 
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.22 KDa
Source organism: Synechococcus sp. CC9311
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0I9X8 (Residues: 1-182; Coverage: 100%)
Gene name: sync_1539
Sequence domains: Ferritin-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: F432
Unit cell:
a: 176.92Å b: 176.92Å c: 176.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bacterial iron detoxification at the molecular level.
Bradley et al. (2020)
1 more

1 mention without citation

Reaction of O2 with a diiron protein generates a mixed-valent Fe2+/Fe3+ center and peroxide.
Bradley et al. (2019)