› 5oxa

X-ray diffraction
1.16Å resolution

Structure of the S205A mutant of the Cyan Fluorescent Protein Cerulean at pH 7.0

Released:
DOI: 10.2210/pdb5oxa/pdb
PDB entry 5oxa coloured by chain, front view.
PDB entry 5oxa coloured by chain, side view.
PDB entry 5oxa coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Chromophore Isomer Stabilization Is Critical to the Efficient Fluorescence of Cyan Fluorescent Proteins.
Gotthard G, von Stetten D, Clavel D, Noirclerc-Savoye M, Royant A
Biochemistry 56 6418-6422 (2017)
PMID: 29148725

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.78 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand B2H 1 x B2H

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 50.96Å b: 63.32Å c: 69.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.116 0.139
Expression system: Escherichia coli BL21

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Citations

1 review citation

Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.
Di Costanzo et al. (2021)
5 other articles