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5oxv

X-ray diffraction
6.72Å resolution

Structure of the 4_601_157 tetranucleosome (C2 form)

Released:
Model geometry
Fit model/data
Source organism: Xenopus laevis
Primary publication:
Capturing Structural Heterogeneity in Chromatin Fibers.
J Mol Biol 429 3031-3042 (2017)
PMID: 28893533

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-119119 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H2B 1.1 Chains: D, H, N, R
Molecule details ›
Chains: D, H, N, R
Length: 126 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 1-126; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H2A type 1 Chains: C, G, M, Q
Molecule details ›
Chains: C, G, M, Q
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H4 Chains: B, F, L, P
Histone H3.2 Chains: A, E, K, O
DNA STRAND 1 (601-based sequence model) Chain: J
DNA STRAND 2 (601-based sequence model) Chain: I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 348.839Å b: 63.274Å c: 271.361Å
α: 90° β: 124.74° γ: 90°
R-values:
R R work R free
0.321 0.317 0.352
Expression system: Escherichia coli