› 5r4s

X-ray diffraction
1.61Å resolution

PanDDA analysis group deposition -- Crystal Structure of HUMAN CLEAVAGE FACTOR IM in complex with EN08775-45

Released:
DOI: 10.2210/pdb5r4s/pdb
PDB entry 5r4s coloured by chain, front view.
PDB entry 5r4s coloured by chain, side view.
PDB entry 5r4s coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kidd SL, Mateu N, Talon R, Krojer T, Aimon A, Bradley AR, Fairhead M, Diaz-Saez L, Sore HF, Madin A, Huber KVM, von Delft F, Spring DR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cleavage and polyadenylation specificity factor subunit 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 197 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43809 (Residues: 33-227; Coverage: 86%)
Gene names: CFIM25, CPSF25, CPSF5, NUDT21
Sequence domains: Nucleotide hydrolase

Ligands and Environments

3 bound ligands:
Ligand RWD 1 x RWD
Ligand ZN 6 x ZN
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3221
Unit cell:
a: 58.91Å b: 58.91Å c: 212.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.209 0.248
Expression system: Escherichia coli

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