› 5suk

X-ray diffraction
2.88Å resolution

G6P bound activated state of yeast glycogen synthase 2

Released:
DOI: 10.2210/pdb5suk/pdb
PDB entry 5suk coloured by chain, front view.
PDB entry 5suk coloured by chain, side view.
PDB entry 5suk coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Redox Switch for the Inhibited State of Yeast Glycogen Synthase Mimics Regulation by Phosphorylation.
Mahalingan KK, Baskaran S, DePaoli-Roach AA, Roach PJ, Hurley TD
Biochemistry 56 179-188 (2017)
PMID: 27935293

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 725 amino acids
Theoretical weight: 82.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P27472 (Residues: 1-705; Coverage: 100%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase

Ligands and Environments

1 bound ligand:
Ligand G6P 6 x G6P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 191.984Å b: 204.562Å c: 205.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.26
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Discovery and Development of Small-Molecule Inhibitors of Glycogen Synthase.
Tang et al. (2020)
2 more

2 mentions without citation

Redox Switch for the Inhibited State of Yeast Glycogen Synthase Mimics Regulation by Phosphorylation.
Mahalingan et al. (2017)
1 more