› 5sv0

X-ray diffraction
2.6Å resolution

Structure of the ExbB/ExbD complex from E. coli at pH 7.0

Released:
DOI: 10.2210/pdb5sv0/pdb
PDB entry 5sv0 coloured by chain, front view.
PDB entry 5sv0 coloured by chain, side view.
PDB entry 5sv0 coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
Structural insight into the role of the Ton complex in energy transduction.
Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK
Nature 538 60-65 (2016)
PMID: 27654919

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biopolymer transport protein ExbB Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 244 amino acids
Theoretical weight: 26.34 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABU7 (Residues: 1-244; Coverage: 100%)
Gene names: JW2974, b3006, exbB
Sequence domains: MotA/TolQ/ExbB proton channel family

Ligands and Environments

2 bound ligands:
Ligand EPE 3 x EPE
Ligand CA 2 x CA
1 modified residue:
Ligand MLY 10 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 137.166Å b: 104.779Å c: 149.364Å
α: 90° β: 91.77° γ: 90°
R-values:
R R work R free
0.212 0.211 0.258
Expression system: Escherichia coli

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Citations

19 review citations

Bacterial outer membrane constriction.
Egan AJF. (2018)
18 more

8 mentions without citation

Structure and Stoichiometry of the Ton Molecular Motor.
Celia et al. (2020)
7 more