› 5sv1

X-ray diffraction
3.5Å resolution

Structure of the ExbB/ExbD complex from E. coli at pH 4.5

Released:
DOI: 10.2210/pdb5sv1/pdb
PDB entry 5sv1 coloured by chain, front view.
PDB entry 5sv1 coloured by chain, side view.
PDB entry 5sv1 coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
Structural insight into the role of the Ton complex in energy transduction.
Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK
Nature 538 60-65 (2016)
PMID: 27654919

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142213 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Biopolymer transport protein ExbB Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 244 amino acids
Theoretical weight: 26.34 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABU7 (Residues: 1-244; Coverage: 100%)
Gene names: JW2974, b3006, exbB
Sequence domains: MotA/TolQ/ExbB proton channel family
Biopolymer transport protein ExbD Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 58 amino acids
Theoretical weight: 6.42 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABV2 (Residues: 1-49; Coverage: 35%)
Gene names: JW2973, b3005, exbD
Sequence domains: Biopolymer transport protein ExbD/TolR

Ligands and Environments

1 bound ligand:
Ligand HG 10 x HG
1 modified residue:
Ligand MLY 10 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 104.942Å b: 196.748Å c: 210.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.255 0.3
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Heme Uptake and Utilization by Gram-Negative Bacterial Pathogens.
Richard et al. (2019)
21 more

4 mentions without citation

Molecular Evolution of Transition Metal Bioavailability at the Host-Pathogen Interface.
Antelo et al. (2021)
3 more