› 5sv2

X-ray diffraction
1.31Å resolution

Toxin VapC21 from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb5sv2/pdb
PDB entry 5sv2 coloured by chain, front view.
PDB entry 5sv2 coloured by chain, side view.
PDB entry 5sv2 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of VapC21 from Mycobacterium tuberculosis at 1.31 Å resolution.
Jardim P, Santos IC, Barbosa JA, de Freitas SM, Valadares NF
Biochem Biophys Res Commun 478 1370-5 (2016)
PMID: 27576202

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease VapC21 Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WF91 (Residues: 1-138; Coverage: 100%)
Gene names: Rv2757c, vapC21
Sequence domains: PIN domain
Structure domains: 5'-nuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: C2
Unit cell:
a: 61.339Å b: 44.351Å c: 56.53Å
α: 90° β: 101.72° γ: 90°
R-values:
R R work R free
0.143 0.142 0.16
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

System-Wide Analysis Unravels the Differential Regulation and In Vivo Essentiality of Virulence-Associated Proteins B and C Toxin-Antitoxin Systems of Mycobacterium tuberculosis.
Agarwal et al. (2018)
4 more

5 mentions without citation

Structural conservation of the PIN domain active site across all domains of life.
Senissar et al. (2017)
4 more