› 5swh

X-ray diffraction
2.5Å resolution

c-Src V281C kinase domain in complex with Rao-IV-151

Released:
DOI: 10.2210/pdb5swh/pdb
PDB entry 5swh coloured by chain, front view.
PDB entry 5swh coloured by chain, side view.
PDB entry 5swh coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
A combined approach reveals a regulatory mechanism coupling Src's kinase activity, localization, and phosphotransferase-independent functions
Register AC, Maly DJ
Mol. Cell (2019)

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.73 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 252-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 71D 2 x 71D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P1
Unit cell:
a: 41.819Å b: 64.139Å c: 74.91Å
α: 77.94° β: 89.5° γ: 89.83°
R-values:
R R work R free
0.244 0.241 0.287
Expression system: Escherichia coli

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